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zymogram renaturing buffer (Bio-Rad)

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Bio-Rad zymogram renaturing buffer
Zymogram Renaturing Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/renaturation+buffer/Zymogram+Sample+Buffer/pmc12358354-74-3-24
Average 94 stars, based on 267 article reviews

zymogram renaturing buffer - by Bioz Stars, 2026-09

94/100 stars

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Incubation:

Article Title: Glutathione has cell protective and anti-catabolic effects in articular cartilage without impairing the chondroanabolic phenotype
Article Snippet: .. Afterwards, the gel was incubated in renaturation buffer (Bio-Rad) for 30 min at RT and subsequently incubated overnight in development buffer according to the Bio-Rad recipe [50 mM Tris-HCl (Roth), 200 mM NaCl (AppliChem), 5 mM CaCl 2 (Merck), 0.02 % Brij-35 (Merck)] at 37 °C. .. The gel was stained using Coomassie R250 Brilliant Blue (Fluka Chemika) staining solution and pro MMP-2 and active MMP-2 bands were revealed by incubation in a de-staining buffer [ , ].

Article Title: Exendin-4 Preserves Blood-Brain Barrier Integrity via Glucagon-Like Peptide 1 Receptor/Activated Protein Kinase-Dependent Nuclear Factor-Kappa B/Matrix Metalloproteinase-9 Inhibition After Subarachnoid Hemorrhage in Rat
Article Snippet: About 40 μl of the sample was loaded per lane onto a polyacrylamide gel containing 0.1% gelatin (Sigma-Aldrich) for electrophoresis. .. Then, the gel was washed in renaturation buffer (Bio-Rad Laboratories) for 1 h. After rinsing with distilled water 10 times, the gel was incubated in development buffer (Bio-Rad Laboratories) at 37°C for 48 h. Staining was performed using Coomassie Brilliant Blue R-250 staining solution (Bio-Rad Laboratories) for 1 h and followed by a destaining solution (10% acetic acid and 40% methanol). ..

Article Title: Hypoxia Inducible Factor-1 α Regulates the Migration of Bone Marrow Mesenchymal Stem Cells via Integrin α 4
Article Snippet: The conditioned medium was separated by 12% SDS-PAGE supplemented with 1 mg/mL of gelatin (Bio-Rad Laboratories). .. The separated proteins were incubated for 30 minutes using a renaturation buffer (Bio-Rad Laboratories), rinsed, and incubated in a development buffer (Bio-Rad Laboratories) at 37°C for 24 hours. ..

Staining:

Chromogenic Assay:

Article Title: Evolution of Primary Hemostasis in Early Vertebrates
Article Snippet: .. The gel was washed to remove SDS and renatured for 1 hr with renaturation buffer (Bio-Rad Laboratories Inc., Hercules, CA) before performing the in-gel chromogenic assay as described above. ..